Lambert Instruments Launches FLIM for Beginners Guide

8th Oct, 2025

Groningen, The Netherlands — October, 2025 — Lambert Instruments has released a new educational resource, FLIM for Beginners, designed to make Fluorescence Lifetime Imaging Microscopy (FLIM) accessible to researchers at all levels.

While traditional fluorescence imaging measures only intensity, FLIM reveals much more — uncovering molecular interactions, metabolic changes, and dynamic processes within living cells. This guide simplifies the concepts behind lifetime imaging and helps scientists get started quickly with practical, real-world insights.

Inside the Guide

  • How FLIM works – Understand why lifetime imaging provides concentration-independent data.

  • Time vs Frequency Domain FLIM – Learn the differences and when to use each method.

  • Phasor analysis made simple – Visualize complex lifetime data at a glance.

  • Real-world applications – Explore uses in metabolism, calcium imaging, FRET, and lifetime unmixing.

  • Getting started with LIFA vTAU – Discover the accessible, camera-based FLIM system from Lambert Instruments.

Whether you’re exploring cell metabolism, protein interactions, or multiplexed imaging, FLIM for Beginners is your starting point for unlocking the full potential of fluorescence lifetime data.

Download the Application Note

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